py arrhenomanes (ATCC)
Structured Review

Py Arrhenomanes, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Comparative Genomics Reveals Insight into Virulence Strategies of Plant Pathogenic Oomycetes"
Article Title: Comparative Genomics Reveals Insight into Virulence Strategies of Plant Pathogenic Oomycetes
Journal: PLoS ONE
doi: 10.1371/journal.pone.0075072
Figure Legend Snippet: Species name, accession numbers, host/substrate and geographical origin of the Pythium strains sequenced in this study.
Techniques Used:
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Figure Legend Snippet: Assembly and annotation statistics
Techniques Used:
Figure Legend Snippet: The predicted proteomes of the seven Pythium species were clustered using OrthoMCL to identify orthologs and close paralogs. The number of gene families shared between the species and total number of clustered genes (numbers in parentheses) are indicated. The numbers outside the Venn diagram show the total number of orthologous clusters and number of genes (in parentheses) within those clusters for each species. Pap, Pythium aphanidermatum; Par, Pythium arrhenomanes ; Pir, Pythium irregulare ; Piw, Pythium iwayamai ; Puls, Pythium ultimum var. sporangiiferum ; Pult, Pythium ultimum var. ultimum ; Pve, Pythium vexans.
Techniques Used:
Figure Legend Snippet: The CAZymes coding genes were annotated using the CAZymes Analysis Toolkit- CAT according to the CAZy database in combination with protein family domain analyses. Gene families absent in at least 2 species are underlined. Comparison of total CAZymes from different classes is listed in . CE, carbohydrate esterase; GH, glycoside hydrolase; GT, glycosyl transferase; PL, polysaccharide lyase; Pap, Pythium aphanidermatum ; Par, Pythium arrhenomanes ; Pir, Pythium irregulare ; Piw, Pythium iwayamai ; Pult, Pythium ultimum var. ultimum ; Puls, Pythium ultimum var. sporangiiferum ; Pve, Pythium vexans ; Phin, Phytophthora infestans ; Phrm, Phytophthora ramorum ; Phsj, Phytophthora sojae ; Hpa, Hyaloperonospora arabidopsidis ; Thps, Thalassiosira pseudonana ; Phtr, Phaeodactylum tricornutum .
Techniques Used: Comparison
Figure Legend Snippet: (A) The typical architecture of a YxSL[RK] effector candidate inferred from 141 sequences from seven Pythium species, Phytophthora infestans , and Phytophthora sojae . The consensus sequence pattern of the YxSL[RK] motif was calculated using WebLogo . The bigger the letter, the more conserved the amino acid site. Please note that the numbers in the sequence logo refer to the corresponding positions in the alignment and thus differ from the average position of the motifs. (B) The YxSL[RK] motif distribution in the proteomes of Pythium species, Phytophthora infestans and Phytophthora sojae is shown. The YxSL[RK] sequence is over-represented in the secretome of Pythium and Phytophthora species relative to the non-secreted proteome ( P ≤0.05). The YxSL[RK] motifs were counted only if they were within the first 30 to 150 residues from the signal peptide. The frequency was calculated as percentage of either all secreted proteins or all non-secreted proteins. Pult, Pythium ultimum var. ultimum ; Puls, Pythium ultimum var. sporangiiferum ; Pir, Pythium irregulare ; Piw, Pythium iwayamai ; Par, Pythium arrhenomanes ; Pap, Pythium aphanidermatum ; Pve, Pythium vexans ; Phin, Phytophthora infestans ; Phrm, Phytophthora ramorum ; Phsj, Phytophthora sojae ; Hpa, Hyaloperonospora arabidopsidis . (C) The typical architecture of an LxLYLAR/K effector motif inferred from 129 sequences from 7 Pythium species. The consensus sequence pattern of the LxLYLAR/K motif was calculated using WebLogo . The bigger the letter, the more conserved the amino acid site. Please note that the numbers in the sequence logo are referring to the corresponding positions in the alignment and thus differ from the average position of the motifs. (D) Number of CRN effector proteins in oomycetes. The number of candidate CRN effectors estimated by Hidden Markov Model (HMM) searches in combination with two other computational methods is shown. The number of CRN effectors from Pythium ultimum var. ultimum , Phytophthora species and H. arabidopsidis were taken from published genome datasets , , , . Pult, Pythium ultimum var. ultimum ; Puls, Pythium ultimum var. sporangiiferum ; Pir, Pythium irregulare ; Piw, Pythium iwayamai ; Par, Pythium arrhenomanes ; Pap, Pythium aphanidermatum ; Pve, Pythium vexans ; Phin, Phytophthora infestans ; Phrm, Phytophthora ramorum ; Phsj, Phytophthora sojae ; Hpa, Hyaloperonospora arabidopsidis .
Techniques Used: Sequencing
Figure Legend Snippet: The circle is a graphical representation of the selected regions from Pythium arrhenomanes (contigs 8, 17, 26, 41, 68, 131, 170, 285, 707) Pythium irregulare (contigs 28, 92, 103, 106, 119, 123, 129, 132, 140, 163, 195, 226, 372, 396), Pythium aphanidermatum (scaffolds 4, 6, 23, 80, 88, 96, 115, 150, 327), Pythium iwayamai (contigs 18, 28, 29, 61, 235), Pythium ultimum var. sporangiiferum (contigs 4, 6, 34, 106, 121, 134, 150, 173, 181, 222, 231, 257, 319, 404, 437, 458, 533, 726), Pythium vexans (contigs 9, 31,42, 94, 151, 160, 209, 220, 347), Phytophthora infestans (supercontig 1.2), Hyaloperonospora arabidopsidis (scaffolds 5, 6, 7, 8, 9) and Thalassiosira pseudonana (chromosome 3). Numbers along each ideogram are sequence lengths in kbp. Syntenic regions were identified through reciprocal best matches between gene models and block identification using MCscan . Each line radiating from Py. ultimum var. ultimum (scf1117875581354) links a syntenic gene pair. Each species is represented by a genus-species abbreviation and colored as Pythium ultimum var. ultimum (Pult) in blue, Pythium arrhenomanes (Par) in orange, Pythium irregulare (Pir) in yellow, Pythium aphanidermatum (Pap) in dark brown, Pythium iwayamai (Piw) in green, Pythium ultimum var. sporangiiferum (Puls) in dark red, Pythium vexans (Pve) in purple, Phytophthora infestans (Phin) in brick red, Hyaloperonospora arabidopsidis (Hpa) in olive green, and Thalassiosira pseudonana (Thaps) in light purple.
Techniques Used: Sequencing, Blocking Assay